Central Analytical Research Facility (CARF)

CARF New Project Form

This form is for new CARF clients to provide information on the proposed project scope, sample declaration, and to select labs/techniques/analyses of interests in preparation for a kick-off meeting.

* Information required in this form:

A) Contact details of you and your nominated supervisor, estimated start date and end date.
B) Account details (QUT account code for staff and student, ABN for non-QUT user)
C) Risk assessment information (HSE HUB Risk Assessment or MAPS # if applicable)
D) Project title and a brief summary
E) Sample declaration - chemical and biological samples
F) Laboratory / Technique / Analysis Selection
G) Working in CARF agreement acknowledgement (please read carefully)

* You can save the form (by copying the url) and complete it later

      * Workflows:

    1)  Upon submission, you will receive a confirmation email for your record.
2) Supervisor review and approval.
3) CARF review and approval.
4) If required, the form may be returned to you for further editing and resubmission.
5) Upon approval, CARF staff will contact you to arrange a kick-off meeting with you and your supervisor.

Please follow up with your Supervisor first before
contacting CARF to ensure the approval workflows run properly.
Thank you.

Privacy Notice:
Please note that this form uses Snapforms, an Australian-based online form provider that stores the data in Australia and adhere to the Australian Privacy Principles. The information provided in this form will only be used for the purpose of registering your interests in CARF as a new client so that a kick-off meeting can be arranged with CARF staff and your supervisor to discuss your project proposal in more details. The information provided will be stored securely by CARF and will not be shared with any third party. If you have any privacy concerns, please contact CARF@qut.edu.au.

P.S. You will receive emails from "Snapforms Notifications" throughout the workflows.

A. Your details

First Name *

Last Name *

Are you a QUT staff or student? *

YesNo

Staff Username or Student Number *(For QUT clients only)

Organisation /Faculty /School (non-QUT) *

Organisation Address (non-QUT) *(no PO box)

Please select your school (Engineering) *

Architecture and Built EnvironmentCivil and Environmental EngineeringElectrical Engineering and RoboticsMechanical, Medical and Process Engineering

Please select your school (Science) *

Your Position Title *

Other Position Title - please specify

Mobile Phone *

Institutional E-mail *

Primary Supervisor Full Name *

Primary Supervisor E-mail *

Project Title *

Estimated Start Date *

Estimated End Date *

B. Account details

At the kick-off meeting, CARF staff will discuss the project costs, if applicable, with you and your project leader or supervisor. If required, a costing proposal will be provided for your approval before any work commences.

Please contact mailto:carf@qut.edu.au for indicative pricing and conditions of access.

Is this project industry funded? (select 'yes' for projects with commercial turnaround time) *

YesNo

QUT Account code *(QUT clients only) - If unsure, please check with your supervisor.

ABN number(non-QUT clients only)

C. Risk Assessment

Select applicable risk assessment for your projects *

Projects with QUT HSE Hub Risk Assessment or MAPS (previously SEF and IHBI projects)All other projects - must attach risk assessment SOPs

HSE HUB Risk Assessment # (or MAPS) *

Please attach details of project risk assessment and any approved standard operating procedures you will use in your project. *

Browse

D. Project Summary

Briefly state your project goals and research question and how your project relates to CARF facilities. *

E-1. Sample Declaration - Chemical Material

I declare that the sample contains chemical material *

YesNo

The term ‘chemical’ includes, but is not limited to, a pure element, a compound of a number of elements, or a mixture of two or more such compounds, and any solution, suspension, fume or aerosol thereof. Chemical materials also include rocks, minerals, oils, soils (which is also a biological material).

Section 1. Chemical Material

All work with hazardous chemicals must comply with the QUT Chemical Management Procedure.

What is the chemical material? *

In what form is the material? *

Other form of the material: *

How will the chemical material be used? *

What chemicals are in the material? *

Chemical sample disposal type: *

Please specify the sample disposal type. *

Storage and disposal instructions: *Please note that samples cannot be stored in CARF and will be disposed according to the instructions if necessary.

Are any chemicals classified as a hazardous chemical? *

YesNo

Is this chemical material classified as a nano-particulate material? *

YesNo

Please refer to the QUT High Risk Chemicals and Red Flag Guideline and HSE Chemical Management - Annex F - QUT High Risk Chemicals for answering the following questions:

Are any chemicals on the QUT High Risk Chemical List? *

YesNo

Chemicals of Security Concern? *

YesNo

Security Sensitive Ammonium Nitrate? *

YesNo

Cryogenic Liquids? *

YesNo

Radioactive Materials? *

YesNo

Chemical Warfare Agent Precursors? *

YesNo

Prohibited and Restricted Carcinogens? *

YesNo

Drugs and Poisons? *

YesNo

Dangerous Goods? *

YesNo

Illicit Drug Precursors? *

YesNo

Controlled Substances *

YesNo

Do you intend to undertake non-ambient (heating/cryo) XRD techniques on the sample? *

YesNo

If yes, please add yourself to the XRD High Temperature MAPS (10360)

If you have ticked yes to at least one of the above, please provide further information in the format as indicated below or indicate the information is uploaded in the next field. *Format: Chemical Name -- UN No. -- CAS No. -- Form -- Concentration -- Quantity -- DG Class (all required)

Please upload information on other chemical materials for your projects here:

Browse

Scheduled Substances (S2-S9)

Are any materials or items coming into CARF a Scheduled Substances (S2-S9)? *

YesNo

Approval Number # *

List S2- S9 items *

E-2. Sample Declaration - Biological Material

I declare that the sample contains biological material *

YesNo

The term ‘biological material’ includes material of human, animal, microbial or plant origin or derivation.

Section 2. Biological Material

All work with biological material must comply with the QUT University Biosafety Committee (UBC), Animal Ethics Committee (UAEC) or Human Research Ethics Committee (UHREC) conditions of use. Please provide relevant Approval Numbers below.

UAEC Approval No. (if required)

UHREC Approval No. (if required)

What is the biological material? *

How will the biological material be used? *

Fixative and/or inactivation treatment *

Biological sample disposal type *

Please specify biological sample disposal type *

Storage and disposal instructions: *Please note that samples cannot be stored in CARF and will be disposed according to the instructions if necessary.

1. Is the biological material genetically modified (i.e., GMO, GM plant)? ^ *

YesNo

UBC Approval No: *

2. Has the biological material been imported on a Permit with a Condition requiring permanent containment within an approved arrangement site? ^ *

YesNo

Permit No.: *

3. Is the biological material infectious to animals or humans? ^ *

YesNo

4. Was the biological material sourced from outside QUT? *

YesNo

Organisation: *

5. Was the biological material collected from a field site (biosecurity movement restrictions may exist)? *

YesNo

Site Location: *

^ If you answered 'yes' to any of 1, 2 or 3, the material can only be used in GP-R413-422.

Microorganism Risk Group: (* Group 3 and 4 cannot be brought into CARF labs) *

123* 4*

F. Laboratory / Techniques / Analysis Selection

GP - Elements and Isotopes Lab - P624, P814 and R431

(Please select at least one instrument from this lab)

Elemental Analysis: CNS or CHNS and O

Solid - CNS only (plants min. 1g; soil min. 3g)Solid - CHNS and O (up to 10 mg)Water - TOC, IC, and TN

Major and Trace Elements

Solid – without digestion: LA-ICPMSSolid – microwave or hotplate acid total digestion or partial extractionLiquid and digested/extracted solids: ICPOES (ppm – ppb range)Liquid and digested/extracted solids: ICPMS (ppb – ppt range)

Gas Analysis

Volatile gas analysis: SIFT-MSGC gas analysis (including Greenhouse gas)

Nutrient and Anion Analysis: F - , Cl - , Br - , Nitrate, Nitrite, Ammonia, etc.

Colorimetric analysis (Gallery discrete analyser)IC (filtered/unfiltered water) or Combustion IC for solids

Stable Isotopes Analysis

15N and/or 13C in solids by EA-IRMS (pre weighed)15N and/or 13C in solids by EA-IRMS (raw or unprepared)15N in N2, N2O or 13C in CO2 Gas (by CryoPrep)H/D, 18O - LGR water isotope analyser

Sample Preparation and Other Analysis

Water quality (pH, alkalinity, conductivity, etc.)Moisture content, ashing or loss on ignitionTitrationSample extraction: DIONEX ASEFreeze drying or oven dryingClean room for ultra-trace analysis sample preparation (R426)

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, certified reference material (CRM), Quality control samples, calibration standards, etc.)

GP - Genomics Lab - R413 – 422

(Please select at least one instrument from this lab)

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

GP - Microscopy Lab - P620, P615, M117

(Please select at least one instrument from this lab)

Optical microscope

Confocal

Helium Ion Micr. (Zeiss Orion NanoFab)

Plasma FIB

SEM (scanning electron microscopy)

High vacuumVariable pressureEnergy dispersive spectroscopy

TEM (transmission electron microscopy)

Morphology / StructureDiffractionMicroanalysis

EPMA (Electron Probe MicroAnalyser)

Wavelength dispersive spectrometry (WDS)Spectroscopic cathodoluminescence (CL)WDS mapping (quant. Or qual.)

FIB (focused ion beam)

Lamella preparationCross-section / Tomography

Sample Preparation

SEM sample preparationThin section sample preparationSample crushing and grinding

Other details(e.g. estimated # of samples, sample preparation requirements, including # of analyses or maps per sample where relevant (e.g. EPMA) etc.)

GP - Physical & Mechanical Properties Lab - P614

(Please select at least one instrument from this lab)

Thermal Analysis

Simultaneous thermal analysis (TGA/DSC) to 1600°CDifferential Scanning Calorimetry (-85°C to 600°C)Thermal diffusivity and conductivity by laser flash analysisLinear thermal expansionOxidation Induction time/temperature

Particle and Surface Analysis

Particle sizing-micron scale (laser diffraction) Particle sizing-nano scale (DLS)Zeta potentialSurface area and porosity (gas adsorption)

Other details(e.g., estimated number of samples, sampling technique, sample preparation requirements, etc.):

GP - Proteomics and Molecular Mass Spectrometry - P636 & P824

(Please select at least one instrument from this lab)

Type of Instrument

Liquid Chromatography - Mass Spectrometry (LC-MS)Gas Chromatography - Mass Spectrometry (GC-MS)ProteomicsLipidomics

Type of Analysis (please select one) *

QualitativeQuantitative

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

GP - Surface Analysis

(Please select at least one instrument from this lab)

Atomic Force Microscopy – Bruker IconAtomic force or scanning tunnelling microscopy in vacuum (high resolution atomic- scale imaging by Omicron UHV-AFM/XA system)Photoelectron spectroscopy (XPS/UPS) (surface chemistry, oxidation states, electronic energy levels, chemically resolved imaging by Kratos AXIS Supra)Ellipsometry (non-destructive optical film analysis)Surface Profilometry (Dektak)TOF-SIMSQuartz Crystal MicrobalanceDrop Shape Analysis

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

GP - Magnetic Resonance Lab - M112

(Please select at least one instrument from this lab)

Nuclear Magnetic Resonance (NMR) Spectroscopy

Bruker 600 MHz NMR with BBO (broadband) probe and SampleJet sample handling (Automated 1D and 2D spectroscopy under ambient conditions)Bruker 400 MHz NMR with QNP (1H, 13C, 15N, 31P) or BBI (broadband) probe (Solution-state 1D and 2D spectroscopy under ambient or VT conditions)Bruker 400 MHz NMR with HRMAS probe for gel-state samples

Electron Paramagnetic Resonance (EPR) Spectroscopy

Other details(e.g., nuclei of interest, specialized protocols, sample types, previous experience etc.)

GP - X-Ray and Particles Lab - P629 and P630

(Please select at least one instrument from this lab)

Please estimate # of samples and indicate any specific analysis requirements, e.g. micro, in vacuo, cryo:Please note: most X-ray analyses may require the user to obtain a Qld Health Radiation Use License, or to arrange for a CARF Technologist to undertake the analysis on a fee-for-service basis. Contact X-ray laboratory staff for further details.

GP - Vibrational Spectroscopy

(Please select at least one instrument from this lab)

Infrared spectroscopy (FT-IR)

Raman spectroscopy

KG - Histology Lab - Q519 & Q520

(Please select at least one instrument from this lab)

Rapid decalcification

Cryo histology

Histomorphometry

Paraffin Histology

Sample preparation (grossing; processing and embedding)Sectioning

Resin Histology

Sample preparation (grossing; processing and embedding)Thin sectioning (Sledge microtomy)Thick sectioning (EXAKT kit)Ground sectioning (EXAKT kit)Lapping & Polishing (Tegra kit)

Staining

General histochemical stainsAutomated immunohistochemical stains: IHC/IF/ISH/FISH/single plexing/ multiplexing

Imaging

MacroimagerStereomicroscopy (brightfield, fluorescence)Optical microscopy (brightfield; fluorescence; polarised light; phase contrast)High throughput Slide scanning (brightfield, fluorescence, z stacks)Tiling

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

KG - Flow Cytometry Lab - Q402

(Please select at least one instrument from this lab)

Flow CytometersFluorescence Activated Cell Sorter

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

KG - Microscopy Lab - Cell Analysis Facility, Q Block levels 4, 6 and 7

(Please select at least one instrument from this lab)

ConfocalBench-top Scanning Electron Microscope

Optical Microscopes

BrightfieldFluorescencePhase Contrast; DICTile Scan; Structured illumination; Deconvolution; z-stacks

Live- Cell Imaging

Label- Free (Phi Holomonitors M4)Fluorescence

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

KG - Spatial Genomics Lab - Q402

(Please select at least one instrument from this lab)

Nanostring Digital Spatial ProfilerNanostring nCounter platform

Other details(e.g., estimated # of samples, sampling technique, sample preparation requirements, etc.)

G. Working In CARF Agreement

Please read this section carefully.

You are bound to this agreement with your acknowledgement. Your supervisor will be notified of any breaches of this agreement. Failure to comply may result in access suspension. Thank you for your attention.

General access

All CARF users must follow QUT Health, Safety and Environment (HSE) policies and procedures.
All CARF users must complete the following mandatory QUT training:
- General Evacuation training annually,
- Health, Safety and Environment induction every two years, and
- Laboratory Safety every two years.

For QUT staff or students, it is their Supervisor’s responsibility to ensure these modules are completed as required.

CARF encourages users to complete ‘Biosafety for PC2’ training every three years, even if they are not using biological or OGTR materials, themselves.
CARF users may only enter laboratories they are trained and inducted by CARF staff to use , unless accompanied by a CARF staff member.
Additional lab-specific mandatory training modules may be required before user access can be granted.
A user’s swipe card or laboratory key must not be used by another person. Do not let someone else enter a CARF lab with you under your lab access.
Do not remove anything (e.g. chemicals, equipment, supplies) from rooms without the permission of the responsible CARF staff member.
Access to CARF may be suspended or terminated in the event of serious neglect, damage, or inappropriate use of equipment or samples, neglect of HSE procedures, or allowing unauthorised people into CARF as follows:
- First time – written warning (email) to CARF user and their supervisor
- Second time – written warning (email) to CARF user and their supervisor
- Third time – access suspended until face-to-face meeting between CARF staff, CARF user and their supervisor.
  
  In cases where people or equipment have been placed at significant risk or harmed, CARF staff have the right to immediately suspend a user’s access without warning.
After-hours access is not guaranteed and only permitted in certain laboratories. Consultation and training with the relevant CARF staff and specific risk assessment regarding the work to occur after-hours must first be approved.

Yes, I acknowledge the above. *

Training and equipment usage

CARF staff are responsible for all laboratory and instrument training. Do not teach, demonstrate or book equipment to/for others.
All CARF users must follow specifications of risk assessments and standard operating procedures for all operations/equipment used at CARF .
CARF staff will not deem a user as competent to use a piece of equipment until sufficient training is completed by the user.
CARF users may only use instruments that the CARF staff have trained them to use.
CARF users shall not interfere with other users' sample or analysis. Contact the responsible staff member if any booking infringement occurs.
Laboratory computers are not to be modified in any way (e.g., installing software, charging phones, changing settings) or used for any purpose other than what you have been told to use them for.
CARF users must report any faults or irregularities to CARF staff immediately.
Any damage to CARF equipment that results from misuse or user error may be charged back to user or their laboratory group / organisation.

Yes, I acknowledge the above. *

Bookings

CARF users can use the CARF booking system to book equipment, after they have completed training on that equipment.
Cancellations must be made at least 24 hours in advance.
If you are going to be more than 15 minutes late for a booking, please notify the laboratory.
If you are not present within 20 minutes of the start of your booking, the booking will be cancelled and the equipment will be made available for others to use.
If equipment is booked and not used, and CARF has not been notified or given an explanation the following will happen:
- First time – CARF user and supervisor contacted by email to request explanation
- Second time – CARF user and their supervisor contacted by email, and booked times charged to the user’s account
- Third time – access suspended until face-to-face meeting between CARF staff, CARF user and their supervisor. Booked times charged to the user’s account.

Yes, I acknowledge the above. *

Data management

Samples are the responsibility of the user and should be removed from the laboratory or disposed of as soon as practicable after analysis and results are available to the user.
Data remains the user’s property, and the user is responsible for managing and archiving their data. CARF is not responsible for any loss of data.

Yes, I acknowledge the above. *

Acknowledgement and authorship

CARF should be acknowledged in any research output (e.g., publication, presentation, poster) arising from data generated by use of CARF laboratories and equipment. This acknowledgement is appropriate professional courtesy, and enables us to track the impact of CARF infrastructure, which in turn helps to justify continued resourcing for the infrastructure and staff. An appropriate acknowledgement is:

“ This work was enabled by use of the Central Analytical Research Facility (CARF) at the
Queensland University of Technology (QUT).”
Where appropriate contributions have been made, CARF staff should be recognised as co-authors (see QUT authorship Policy at http:// www.mopp.qut.edu.au/D/D_02_06.jsp#D_02_06.08.mdoc).
Please notify carf@qut.edu.au of any research outputs that arise from use of CARF.

Yes, I acknowledge the above. *

Payment

Charges for training and use of CARF infrastructure, if applicable, are agreed between CARF and the User via a quotation for services.

Yes, I acknowledge the above. *

H. Approval Workflows

Thank you. Your form will now be forwarded for approval with workflow steps below:

Supervisor Approval: Once submitted, this form will be forwarded to your nominated Supervisor or Line Manager via email. You will receive an email notification if your submission is approved. If your submission is rejected, it will be returned to you for editing / resubmission.
CARF Approval: Upon approval in Step 1, your submission will then be forwarded to CARF for internal processing and approval. You will receive an email notification if approved. If any revision is needed, the form will be returned to you for further refinement.
Kick-Off meeting: Upon CARF approval, you will be notified by a CARF staff to arrange a kick-off meeting along with your supervisor / line manager to discuss your project in more detail.

Please note that the notification emails will come either from carf@qut.edu.au or Snapforms.
Please contact mailto:carf@qut.edu.au if you have any enquiries.

Supervisor Approval *

ApprovedPending - request further amendment

If you approve this application, please provide and confirm QUT account code for this project if applicable.

Supervisor Comments:

Please provide and confirm QUT account code for this client: *

CARF Approval *

ApprovedPending - request further amendment

CARF Comments

Meeting info - to be filled in during kick-off meeting:

Date of meeting

Kick-off Meeting Participants

CARF Primary Contact

CARF Instrument/Labs

CARF Resources

Client's resources (i.e., standards)

Quote for service provided?

YesNot Required

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